Education & Experience
1988-1991: Ph.D., Molecular Virology, University of Cambridge, UK, with Dr S.C.Inglis
1983-1986: M.S., Medical Microbiology, Sichuan University (formerly West China University of Medical Sciences, with Professor Z.Lin
1878-1981: Diploma, Medical Laboratory Technology, Hubei Polytechnic University (formerly Huangshi Health School)
PROFESSIONAL EXPERIENCE
January 2017-present: Professor, South China Agricultural University, Guangdong Province Key Laboratory Microbial Signals & Disease Co, and Integrative Microbiology Research Centre, Guangzhou 510642, Guangdong, People’s Republic of China
June 2010-August 2018: Associate professor and visiting Professor, School of Biological Sciences, Nanyang Technological University, Singapore
October 2009-August 2017: Guest Professor and PhD Supervisor, Lanzhou Institute of Veterinary Research, Lanzhou, People's Republic of China
September 2003-May 2010: Principal Investigator, Laboratory of Molecular Virology and Viral Pathogenesis, Institute of Molecular and Cell Biology, Singapore
February 2002-September 2003: Associate Professor, School of Biological Sciences, Nanyang Technological University, Singapore
January 1996-January 2002: Principal Investigator, Laboratory of Animal Virology, Institute of Molecular Agrobiology, the National University of Singapore, Singapore
October 1991-January 1996: Postdoctoral research Associate, Department of Medicine, and Department of Pathology, University of Cambridge, Cambridge, United Kingdom
July 1986-September 1988: Teaching Assistant and Lecturer, Department of Microbiology and Immunology, Sichuan University (formerly West China University of Medical Sciences), Chengdu, Sichuan, People's Republic of China
July 1981-August 1983: Medical Microbiologist, Medical Microbiology Laboratory, Huangshi Central Hospital (formerly Huangshi No. 3 Hospital), Huangshi, Hubei, People's Republic of China1.1988-1991: Cambridge Overseas Trust Scholarship
2. 2015, Five Years of Service Award, Ministry of Health, Singapore
Awards & Honors
1. Cambridge Overseas Trust Scholarship
2. 2015, Five Years of Service Award, Ministry of Health, Singapore
Research Fields
Molecular Virology, animal RNA viruses; replication mechanisms and pathogenesis; virus-host interactions; host anti-viral responses, research and development of novel vaccines and diagnostics
Recent Publications (since 2017)
1. To J, Surya W, Fung TS, Li Y, Verdià-Bàguena C, Queralt-Martin M, Aguilella VM, D. X. Liu*, Torres J. (2017). Channel inactivating mutations and their revertant mutants in the envelope protein of the infectious bronchitis virus. Journal of Virology 2017 Feb 14;91(5). pii: e02158-16.
2. Guo H, Huang M, Yuan Q, Wei Y, Gao Y, Mao L, Gu L, Tan YW, Zhong Y, D. X. Liu*, Sun S*. (2017). The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain. PLoS One. 2017 Jan 12;12(1):e0170123. doi: 10.1371/journal.pone.0170123.
3. Yanquan Wei, Yanhong Ji, Huichen Guo, Xiaoying Zhi, Shichong Han, Yuan Gao, Yun Zhang, D. X. Liu*, Shiqi Sun*. (2017). CD59 Association protects Infectious Bronchitis Virus against complement-mediated destruction. Journal of General Virology 98, 2725-2730. 2017 Oct 25. doi: 10.1099/jgv.0.000962. [Epub ahead of print].
4. To Sing Fung and D. X. Liu*. (2017). Activation of the c-Jun NH2-terminal kinase pathway by coronavirus infectious bronchitis virus promotes apoptosis independently of c-Jun. Cell Death and Disease 8, 3215.
5. Jie Zheng, Yoshiyuki Yamada，To Sing Fung, Raymond Chia, Mei Huang, and D. X. Liu*. (2018). Identification of N–linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture. Virology 513, 65-74.
6. Yong Wah Tan, To Sing Fung, Hongyuang Shen, Mei Huang, and D. X. Liu*. (2018). Coronavirus Infectious Bronchitis Virus Non-structural Proteins 8 and 12 Form Stable Complex Independent of the Non-translated Regions of Viral RNA and Other Viral Proteins. Virology 513, 75-84.
7. Ru Ying Puah, Huan Jia, Manikantha Maraswami, Desiree-Faye Kaixin Toh, Rya Ero, Lixia Yang, Kiran M. Patil, Ruimin Sun, Cailing Tong, Mei Huang, Xin Chen, Teck Peng Loh, Yong-Gui Gao, D. X. Liu* and Gang Chen*. (2018). Selective Binding to mRNA Duplex Regions by Chemically Modified PNAs Stimulates Ribosomal Frameshift. Biochemistry 57(1), 149-159.
8. Hui Hui Wong, To Sing Fung, Shouguo Fang, Mei Huang, My Tra Le, and D. X. Liu*. (2018). Severe Acute Respiratory Syndrome Coronvirus Proteins 8b and 8ab Suppress the Interferon Signaling Pathway by Mediating Ubiquitin-Dependent Rapid Degradation of Interferon Regulatory Factor 3. Virology 515, 165-175.
9. To Sing Fung and D. X. Liu*. (2018). Post-translational modifications of coronavirus proteins: roles and function. Future Virology 13, 405-430. Published online: 21 May 2018.
10. Si Min Zhang, Ying Liao, Tuan Ling Neo, Yanning Lu, D. X. Liu, Anders Vahlne, James P. Tam*. (2018). Identification and Application of Self-binding Zipper-like Sequences in SARS-CoV Spike Protein. International Journal of Biochemistry and Cell Biology 2018 Aug;101:103-112. doi: 10.1016/j.biocel.2018.05.012. Epub 2018 May 22.
11. Hongliu An, Zhichao Cai, Yuying Yang, Zhaoxiong Wang, D. X. Liu*, Shouguo Fang*. (2019). Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus. Virology 528, 176-180.
12. Jia Qi Liang, Shouguo Fang, Quan Yuan, Mei Huang, Rui Ai Chen, To Sing Fung*, D. X. Liu*. (2019). N-Linked Glycosylation of the Membrane Protein Ectodomain Regulates Infectious Bronchitis Virus-Induced ER Stress Response, Apoptosis and Pathogenesis. Virology 531, 48-56.
13. To Sing Fung and D. X. Liu*. (2019). The ER stress sensor IRE1 and MAP kinase ERK modulate autophagy induction in cells infected with coronavirus infectious bronchitis virus. Virology 533，34-44.
14. To Sing Fung and D. X. Liu*. (2019). Human Coronavirus: Host-Pathogen Interaction. Annual Review of Microbiology 73, 529-557.
15. Liu DX*, Ng YL, Fung TS. Infectious Bronchitis Virus. In Avian Virology: Current Research and Future Trends. (Ed. S. K. Samal). 133-178. Caister Academic Press. 2019; https://doi.org/10.21775/9781912530106.05.
16. Shumin Li, Lixia Yuan, Guo Dai, Rui Ai Chen, D. X. Liu* and To Sing Fung*. (2020). Regulation of the ER Stress Response by the Ion Channel Activity of the Infectious Bronchitis Coronavirus Envelope Protein Modulates Virion Release, Apoptosis, Viral Fitness, and Pathogenesis. Frontiers in Microbiology 10, 3022.
17. Chengwei Xiang, Mei Huang, Ting Xiong, Fang Rong, Linyu Li, D. X. Liu* and Rui Ai Chen*. (2020). Transcriptomic Analysis and Functional Characterization Reveal the Duck Interferon Regulatory Factor 1 as an Important Restriction factor in the Replication of Tembusu Virus. Frontiers in Microbiology 11, 2069.
18. Xiao Ying Liang, Qing Chun Zhu, Jia Qi Liang, Si Ying Liu, D. X. Liu* and To Sing Fung*. (2020). Development of HiBiT-tagged recombinant infectious bronchitis coronavirus for efficient in vitro and in vivo viral quantification. Frontiers in Microbiology 11, 2100.
19. Nan Nan Wang, Mei Huang, Qiong Luo, Jun Xian Yie, Qianru Du, D. X. Liu* and Rui Ai Chen*. (2020). Rapid Development of an Efficacious Vaccine Candidate by Construction of an Infectious Cloning System and Attenuation of a Genotype VII Velogenic Newcastle Disease Virus Isolate. Frontiers in Veterinary Science 7, 648.
20. Liu DX*, Ng YL, Fung TS. Coronaviruses as vaccine vectors for veterinary pathogens. In Viral Vectors in Veterinary Vaccine Development: A Textbook (Ed. Thiru Vanniasinkam, Suresh Tikoo and Siba Samal). 149-168. Springer Nature 2020. https:doi.org/10.1007/978-3-030-51927-8_10.
21. Liu DX*, Liang JQ, Fung TS. Human Coronavirus-229E, -OC43, -NL63, and -HKU1. (2020). Reference Module in Life Sciences. 2020: B978-0-12-809633-8.21501-X. PMC | ID: covidwho-827842.
22. Guo Dai, Mei Huang, To Sing Fung* and D. X. Liu*. (2020). Research progress in the development of porcine reproductive and respiratory syndrome virus as a viral vector for foreign gene expression and delivery. Expert Review of Vaccine 19, 1041-1051.
23. Xiao-Yu Zhang, Jing Guo, Xin Wan, Jin-Ge Zhou, Jia Lu, Wei-Ping Jin, Wen-Hui Wang, An-Na Yang, D. X. Liu, Zheng-Li Shi, Zhi-Ming Yuan, Xin-Guo Li, Kai Duan, Sheng-Li Meng, Ze-Jun Wang, Xiao-Ming Yang, Shuo Shen. (2020). Biochemical and antigenic characterization of the structural proteins and their post-translational modifications in purified SARS-CoV-2 virions of an inactivated vaccine candidate. Emerging Microbes & Infections 9, 2653-2662.
24. Li Xia Yuan, Jia Qi Liang, QingChun Zhu, Guo Dai, Shumin Li, To Sing Fung* and D. X. Liu*. (2021). A gammacoronavirus, avian infectious bronchitis virus, and an alphacoronavirus, porcine epidemic diarrhea virus, exploit a cell-survival strategy via upregulation of cFOS to promote viral replication. Journal of Virology 95, e02107-20.
25. To Sing Fung and D. X. Liu*. (2021). Similarities and Dissimilarities of COVID-19 and Other Coronavirus Diseases. Annual Review of Microbiology 75,19-47. https://doi.org/10.1146/annurev-micro-110520-023212.
26. Liu DX*, Liang JQ, Fung TS. Human Coronavirus-229E, -OC43, -NL63, and -HKU1 (Coronaviridae). Encyclopedia of Virology. 2021: 428–440. Published online 2021 Mar 1. doi: 10.1016/B978-0-12-809633-8.21501-X. Guest Editor (s): Dennis H. Bamford and Mark Zuckerman.
27. Jiahao Zhang, Kaixiong Ma, Bo Li, Yiqun Chen, Ziwen Qiu, Jinchao Xing, Jinyu Huang, Chen Hu, Yifan Huang, Huanan Li, D. X. Liu, Ming Liao*, and Wenbao Qi*. (2021). A risk marker of tribasic hemagglutinin cleavage site in influenza A (H9N2) virus. Communications Biology (2021) 4:71 | https://doi.org/10.1038/s42003-020-01589-7 | www.nature.com/commsbio.
28. Huan Wang， D. X. Liu， Yingjie Sun， Chunchun Meng， Lei Tan， Cuiping Song， Xusheng Qiu， Weiwei Liu， Chan Ding， Ying Liao. (2021). Upregulation of DUSP6 impairs infectious bronchitis virus replication by negatively regulating ERK pathway and promoting apoptosis. Veterinary Research 52, 7.
29. Qing Chun Zhu, ShuMin Li, Li Xia Yuan, Rui Ai Chen, D. X. Liu* and To Sing Fung*. (2021). Induction of the proinflammatory chemokine interleukin-8 is regulated by integrated stress response and AP-1 family proteins activated during infectious bronchitis coronavirus infection. International Journal of Molecular Sciences 22, 5646.
30. Shumin Li, Siying Liu, Rui Ai Chen, Mei Huang, To Sing Fung, D. X. Liu* (2022). Activation of the MKK3-p38-MK2-ZFP36 Axis by Coronavirus Infection Restricts the Upregulation of AU-Rich Element-Containing Transcripts in Proinflammatory Responses. Journal of Virology 96 (5) e02086-21.
31. Yu Shi, Xiaobei Yang, Xiaoxin Ye, Jiaying Feng, Tianfang Cheng, Xiaofan Zhou, D. X. Liu, Linghui Xu*, Junxia Wang*. (2022). The methyltransferase HemK regulates the virulence and nu-trient utilization of the phytopathogenic bacterium Xanthomo-nas citri subsp. citri. International Journal of Molecular Sciences 23, 3931.